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實(shí)驗(yàn)室簡(jiǎn)介： 分子酶學(xué)與合成生物學(xué)實(shí)驗(yàn)室依托于上海交通大學(xué)微生物代謝國(guó)家重點(diǎn)實(shí)驗(yàn)室，致力于解析酶催化與分子進(jìn)化規(guī)律，發(fā)展酶定向進(jìn)化技術(shù)體系，并開(kāi)發(fā)酶在工業(yè)、醫(yī)藥等領(lǐng)域的重大創(chuàng)新應(yīng)用。 實(shí)驗(yàn)室承擔(dān)了國(guó)家973計(jì)劃項(xiàng)目、863項(xiàng)目、國(guó)家重大研究計(jì)劃項(xiàng)目、自然科學(xué)基金委重大儀器專項(xiàng)、自然科學(xué)基金等研究任務(wù)。在science adv., nature commun., angew. chem., jacs, anal chem, j. biol chem, 等著名雜志發(fā)表多篇論文。 代表性論文： 1)xi deng, liuqing chen, mohan hei, tiangang liu, yan feng, guang-yu yang. structure-guided reshaping of the acyl binding pocket of 'tesa thioesterase enhances octanoic acid production in e. coli, metab eng. 2020, 61:24-32. 2)yumeng tan, yong zhang, yunbin han, hao liu, haifeng chen, fuqiang ma, stephen g withers, yan feng, guangyu yang. directed evolution of an α1,3-fucosyltransferase using a single-cell ultrahigh-throughput screening method. science advances, 2019, eaaw8451 3)fuqiang ma#, meng ting chung#, yuan yao, robert nidet, lap man lee, allen p. liu, yan feng, katsuo kurabayashi*, guang-yu yang*. efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform. nature communications, 2018, 9:1030. (#equal contribution) 4)hoque ma, zhang y, chen l, yang g, khatun ma, chen h, hao l, feng y*. stepwise loop insertion strategy for active site remodeling to generate novel enzyme functions. acs chemical biology. 2017, 12(5):1188-1193. 5)zhuang y#, yang gy#, chen x, liu q, zhang x, deng z, feng y*. biosynthesis of plant-derived ginsenoside rh2 in yeast via repurposing a key promiscuous microbial enzyme. metabolic engineering. 2017, 42:25-32. (#equal contribution) 6)han yb, chen lq, li z, tan ym, feng y, yang gy*. structural insights into the broad substrate specificity of a novel endo-glycoceramidase i belonging to a new subfamily of gh5 glycosidase. j biol chem. 2017, 292(12):4789-4800. 研究課題 1. 利用結(jié)構(gòu)生物學(xué)、家族進(jìn)化樹(shù)分析、祖先基因重構(gòu)等方法揭示自然界關(guān)鍵酶家族的進(jìn)化規(guī)律，探討酶高效的催化功能與底物選擇性的形成機(jī)制與調(diào)控規(guī)律； 2. 開(kāi)發(fā)蛋白質(zhì)從頭設(shè)計(jì)、微流控酶活性超高通量篩選等酶分子設(shè)計(jì)與定向進(jìn)化的新技術(shù)方法，提升工程化酶人工構(gòu)建與優(yōu)化的效率； 3. 針對(duì)具有重大實(shí)際應(yīng)用需求對(duì)關(guān)鍵酶進(jìn)行酶分子設(shè)計(jì)與定向進(jìn)化，提高酶對(duì)非天然底物的選擇性、催化效率及其對(duì)環(huán)境的適應(yīng)性，促進(jìn)酶在生物醫(yī)藥領(lǐng)域的應(yīng)用。